Whakaahuatanga taipitopito
Ko te kitenga o te antibody wheori wheori hipi he mea tito he microplate kua pania-mua ki te antigen pūmua karihi hipi pox, nga tohu tohu enzyme me etahi atu reagents tautoko, ka whakamahia te kaupapa o te enzyme-linked immunoassay (ELISA) ki te kite i te antibody pox hipi i roto i te tauira serum hipi.I te wa o te whakamatautau, ka taapirihia te serum mana me te tauira hei tirotirohia ki te pereti microplate, a mena kei roto i te tauira he antibody pox hipi i muri i te incubation, ka herea ki te antigen i runga i te pereti microplate, me etahi atu waahanga kaore e herea. ka tangohia i muri i te horoi;Kātahi ka tāpirihia te tohu whākōkī kia herea motuhake ki te matatini antigen-antibody i runga i te pereti microplate;Ko nga tohu o te whanariki kaore i herea ka tangohia ma te horoi, ka tapirihia te otinga TMB substrate ki nga puna, ka hangaia te hua kikorangi e te hohenga o nga conjugates microplate, a he pai te hono o te hohonu o te tae ki te nui o nga antibodies kei roto i te tauira.I muri i te tapiritanga o te otinga whakamutu ki te whakamutu i te tauhohenga, ka kowhai te hua;Ko te uara ngongo o ia puna tauhohenga ka whakatauhia e te kaipanui microplate i te roangaru o te 450 nm ki te whakatau mena kei roto i te tauira nga paturopi hipi.
Ihirangi Whakaritea
Tukatuka Hanganga Whakamatau Rapid Rau Kore i tapahia
